Ultrasound-assisted extraction of anti-inflammatory actives from corn silk (Zea mays L.): Process optimization, machine learning screening, and interaction mechanisms.

ABSTRACT: Inflammation is an important immune response in our organism, the intervention of anti-inflammatory substances is critical to control inflammation, in which Cyclooxygenase-2 (COX-2) serves as a key target to inhibit inflammatory factors. This study employed ultrasonic-assisted extraction (UAE) to prepare isoorientin from corn silk (Zea mays L.), optimizing extraction parameters to achieve a maximum yield of 1.26 mg/g isoorientin under optimal conditions. A quantitative structure-activity relationship (QSAR) model was utilized to screen four potential COX-2 inhibitors, including isoorientin, whose excellent inhibitory activities were validated through in vitro COX-2 inhibition assays. Notably, isoorientin demonstrated significant COX-2 inhibitory potency with an IC₅₀ value of 18.

SUBMITTER: Zhao HF 

PROVIDER: S-EPMC12266474 | biostudies-literature | 2025 Jun

REPOSITORIES: biostudies-literature