Offspring metabolic programming via the maternal diet increases susceptibility to metabolic dysregulation.

ABSTRACT:

Background

Maternal nutrition during critical developmental windows is crucial for offspring metabolic programming. Methionine, an essential amino acid, is crucial in pancreatic differentiation. However, the impact of a maternal methionine-deficiency (MD) diet on offspring during the critical stage of embryonic pancreatic differentiation remains unclear.

Methods

We used an in vitro human induced pluripotent stem cell (hiPSC) differentiation model and in vivo mouse and rat models to assess the impact of short-term maternal MD during pancreatic development. Offspring metabolic outcomes were evaluated under control or high-fat diet conditions. Multi-omics analyses were performed to explore mechanistic pathways, and Ruminococcus flavefaciens supplementation was used to assess mi

SUBMITTER: Li X 

PROVIDER: S-EPMC12266555 | biostudies-literature | 2025 Jun

REPOSITORIES: biostudies-literature