Apical-out bovine intestinal organoids as an infection model for <i>Cryptosporidium parvum</i>.

ABSTRACT: Cryptosporidium parvum is a major pathogen responsible for neonatal calf diarrhoea, but research has been hampered due to the lack of in vitro models that can complete the life cycle. In this scenario, human and murine small intestinal organoids (enteroids) are emerging as new in vitro tools. However, models employing bovine cells, relevant for the pathogenesis in the target species, are lacking. Thus, a panel of bovine enteroids was isolated in this study. Enteroids have an enclosed apical lumen, and the parasite must be delivered to the apical side of the cells to facilitate infection. Two different methods of reversing cell polarity were used to generate bovine apical-out enteroids: dissociation in ethylenediaminetetraacetic acid (EDTA), and dissociation in trypsin.

SUBMITTER: Varegg MS 

PROVIDER: S-EPMC12266560 | biostudies-literature | 2025

REPOSITORIES: biostudies-literature