Inactivation of Histone Chaperone HIRA Unmasks a Link Between Normal Embryonic Development of Melanoblasts and Maintenance of Adult Melanocyte Stem Cells.

ABSTRACT: Evidence indicates that the integrity of in utero development influences late life healthy or unhealthy aging; however, specific links between them are unclear. Histone chaperone HIRA is thought to play a role in both life stages, and here, we explore this role using the murine pigmentary system by investigating and comparing the effects of its lineage-specific knockout, either conditionally during embryogenesis or postnatally. Embryonic knockout of Hira in tyrosinase+ neural crest-derived lineages, including melanoblasts, led to reduced melanoblast numbers during embryogenesis, with single-cell RNA sequencing analysis indicating evidence of lineage-specificity defects. This was supported in an in vitro model using melb-a melanoblasts in which Hira knockdown affected lineage identity and m

SUBMITTER: Jaber-Hijazi F 

PROVIDER: S-EPMC12266762 | biostudies-literature | 2025 Jul

REPOSITORIES: biostudies-literature