Exploring single-molecule interactions: heparin and FGF-1 proteins through solid-state nanopores.

ABSTRACT: Detection and characterization of protein-protein interactions are essential for many cellular processes, such as cell growth, tissue repair, drug delivery, and other physiological functions. In our research, we have utilized emerging solid-state nanopore sensing technology, which is highly sensitive to better understand heparin and fibroblast growth factor 1 (FGF-1) protein interactions at a single-molecule level without any modifications. Understanding the structure and behavior of heparin-FGF-1 complexes at the single-molecule level is very important. An abnormality in their formation can lead to life-threatening conditions like tumor growth, fibrosis, and neurological disorders. Using a controlled dielectric breakdown pore fabrication approach, we have characterized individual heparin

SUBMITTER: Thyashan N 

PROVIDER: S-EPMC12266928 | biostudies-literature | 2024 May

REPOSITORIES: biostudies-literature