Stepwise ATP translocation into the endoplasmic reticulum by human SLC35B1.

ABSTRACT: ATP generated in the mitochondria is exported by an ADP/ATP carrier of the SLC25 family¹. The endoplasmic reticulum (ER) cannot synthesize ATP but must import cytoplasmic ATP to energize protein folding, quality control and trafficking^2,3. It was recently proposed that a member of the nucleotide sugar transporter family, termed SLC35B1 (also known as AXER), is not a nucleotide sugar transporter but a long-sought-after ER importer of ATP⁴. Here we report that human SLC35B1 does not bind nucleotide sugars but indeed executes strict ATP/ADP exchange with uptake kinetics consistent with the import of ATP into crude ER microsomes. A CRISPR-Cas9 cell-line knockout demonstrated that SLC35B1 clusters with the most essential SLC transporters for cell growth, consist

SUBMITTER: Gulati A 

PROVIDER: S-EPMC12267056 | biostudies-literature | 2025 Jul

REPOSITORIES: biostudies-literature