Range extender mediates long-distance enhancer activity.

ABSTRACT: Although most mammalian transcriptional enhancers regulate their cognate promoters over distances of tens of kilobases, some enhancers act over distances in the megabase range¹. The sequence features that enable such long-distance enhancer-promoter interactions remain unclear. Here we used in vivo enhancer-replacement experiments at the mouse Shh locus to show that short- and medium-range limb enhancers cannot initiate gene expression at long-distance range. We identify a cis-acting element, range extender (REX), that confers long-distance regulatory activity and is located next to a long-range limb enhancer of Sall1. The REX element has no endogenous enhancer activity. However, addition of the REX to other short- and mid-range limb enhancers substantially increases their genomi

SUBMITTER: Bower G 

PROVIDER: S-EPMC12267059 | biostudies-literature | 2025 Jul

REPOSITORIES: biostudies-literature