The enzyme kinetics of branched-chain fatty acid synthesis of metazoan fatty acid synthase.

ABSTRACT: Branched-chain fatty acids (BCFAs) exhibit enhanced oxidative stability, lower melting points, and reduced viscosity compared to straight-chain fatty acids (StCFAs), making them valuable for biological systems and industrial applications. Previous studies have shown that metazoan fatty acid synthase (mFAS) can produce BCFAs through the incorporation of branched starter units and branched extender substrates such as methylmalonyl-CoA (metmal-CoA). However, the mechanistic and kinetic underpinnings of BCFA biosynthesis in metazoans remain poorly understood. Here, we address this by characterizing the kinetic parameters of mFAS-catalyzed BCFA synthesis using NADPH consumption assays and analyzing the synthesized products via GC-MS. These experiments revealed a lower turnover number of mFAS wi

SUBMITTER: Gusenda C 

PROVIDER: S-EPMC12267109 | biostudies-literature | 2025 Aug

REPOSITORIES: biostudies-literature