Glutamine potentiates cefoperazone-sulbactam activity against <i>Pseudomonas aeruginosa</i> by increasing membrane permeability and cellular uptake.

ABSTRACT:

Purposes

The combination of an antibiotic with a metabolic reprogramming agent is anticipated to emerge as a promising therapeutic strategy against antibiotic-resistant bacteria, though this hypothesis requires validation through preclinical pharmacodynamic studies.

Methods

This study evaluated the preclinical pharmacodynamic profile of cefoperazone-sulbactam (SCF) combined with glutamine against Pseudomonas aeruginosa clinical isolates, including 54 antibiotic-sensitive (S-PA), 20 multidrug-resistant (MDR-PA), and 185 carbapenem-resistant strains (CR-PA).

Results

The combination demonstrated synergistic efficacy in 251 cases (96.9%), equivalence in 7 (2.7%), and no interaction in 1 (0.4%) compared to SCF monotherapy. Time-kill assays, bacterial load quantifica

SUBMITTER: Xiang J 

PROVIDER: S-EPMC12267172 | biostudies-literature | 2025

REPOSITORIES: biostudies-literature