Ionically crosslinked biohybrid gelatin-based hydrogels for 3D cell culture.

ABSTRACT: The transition from two-dimensional to three-dimensional cell cultures has transformed the understanding of cell physiology and cell-matrix interactions. Extracellular matrix (ECM) mimics tend to fall into either the natural or synthetic categories. Naturally occurring ECM mimics, such as collagen and gelatin, have superior bioactive properties but typically lack tuneability. Conversely, synthetic ECM mimics are highly defined but even with modifications, can lack the bioactivity of natural proteins. Therefore, to take advantage of the potential of both natural and synthetic ECM mimics, a biohybrid ionically crosslinked gelatin hydrogel was synthesised. This was achieved by utilising free amine groups along the gelatin backbone as the basis for a reversible addition - fragmentation chain-t

SUBMITTER: Du EY 

PROVIDER: S-EPMC12267368 | biostudies-literature | 2025

REPOSITORIES: biostudies-literature