CALR promotes corneal epithelial cell proliferation and migration through Wnt7a.

ABSTRACT:

Objective

The repair of corneal epithelial injury is essential to maintain the cornea integrity and transparency, and the molecular regulation mechanism is still unclear. CALR promotes wound healing through a variety of biological effects. Therefore, this study explored effect and mechanism of CALR on corneal epithelial wound healing.

Methods

The model of repairing corneal epithelium injury in mice was established, and corneal epithelial tissues were collected from the model group and the control group. oe-CALR or sh-Wnt7a was transfected into HCE-2[50.B1] cells by Lipofectamine 2000 to over-express CALR or knock down Wnt7a in vitro. CALR mRNA expression was detected by RT-qPCR. CCK-8, clone formation assay, cell senescence, flow cytometry, wound healing and Transwell migrat

SUBMITTER: Wang Q 

PROVIDER: S-EPMC12267377 | biostudies-literature | 2025 Jul

REPOSITORIES: biostudies-literature