Improved Annotation of Internal Fragments via Trapped-Ion Mobility Enhanced Top-Down Sequencing of Protein Ions.

ABSTRACT: Analysis of intact protein ions allows for valuable insights into complex biological processes. However, top-down mass spectrometry data is often convoluted and frequently results in numerous overlapping product ions. Ion mobility spectrometry (IMS) can aid in the deconvolution of these spectra, and we previously demonstrated that the IMS separations provided by trapped IMS (TIMS) significantly increased the sequence coverage provided by collision induced dissociation (CID). In this work, we further improve the "CIDtims" method by incorporating the dynamic control of ion populations, optimizing the bioinformatic approach to better leverage the mobility separation, and finally porting the technique to an improved version of the instrument, the timsTOF Pro2. Lastly, we utilize these improvem

SUBMITTER: Graham KA 

PROVIDER: S-EPMC12267681 | biostudies-literature | 2025

REPOSITORIES: biostudies-literature