TaCIPK19-3D Improves Photosynthetic Machinery, Growth, Yield, and Salt Tolerance in Transgenic Rice.

ABSTRACT: Calcineurin B-like interacting protein kinases (CIPKs) are central regulators of plant development and stress adaptation. However, the specific roles of individual CIPK family members remain largely unexplored in major crops like wheat and rice. In this study, we characterized the function of TaCIPK19-3D through overexpression in transgenic rice and CRISPR-Cas9-mediated oscipk19 knockout lines. Expression profiling and subcellular localization analyses revealed that TaCIPK19-3D is associated with chloroplast development and metabolic activity. Overexpression lines exhibited enhanced chloroplast structure, increased chlorophyll biosynthesis, stomatal conductance, net photosynthetic rate, transpiration, and elevated levels of K⁺/Na⁺, Ca²⁺, and Mg²⁺, resulting in improved growth and yield com

SUBMITTER: Arif M 

PROVIDER: S-EPMC12267765 | biostudies-literature | 2025 Jul

REPOSITORIES: biostudies-literature