COmapper: high-resolution mapping of meiotic crossovers by long-read sequencing in Arabidopsis.

ABSTRACT: Meiotic crossovers rearrange existing genetic variation between homologous chromosomes, profoundly affecting genomic diversity. Crossovers are typically constrained to one to three events per chromosome pair, and their distribution is shaped by chromatin accessibility and DNA polymorphisms. Genome-wide crossover maps can be generated in plants by high-throughput short-read sequencing or linked-read sequencing. Here, we use long-read nanopore sequencing technology to develop a crossover mapping pipeline, COmapper, for high-resolution mapping of genome-wide crossovers from pooled DNA of F₁ hybrid pollen and F₂ recombinant seedlings derived from a cross between Arabidopsis thaliana accessions Col and Ler. We validate the high accuracy of COmapper by applying nanopore lon

SUBMITTER: Byun D 

PROVIDER: S-EPMC12267935 | biostudies-literature | 2025 Aug

REPOSITORIES: biostudies-literature