An Acoustofluidic Device for Sample Preparation and Detection of Small Extracellular Vesicles.

ABSTRACT: Small extracellular vesicles (sEVs) have emerged as powerful vectors for liquid biopsy, offering a noninvasive window into the dynamic physiological and pathological states of the body. However, to fully leverage the clinical potential of sEV biomarkers, it is imperative to develop robust and efficient technologies for their isolation and analysis. In this study, we introduce a novel sharp-edge acoustofluidic platform designed for rapid and effective sample preparation, coupled with sensitive detection of specific sEV populations based on their surface markers. Our approach utilizes acoustically activated sharp-edge microstructures to concentrate bead-bound sEVs within the microfluidic device, facilitating immediate visualization by fluorescence microscopy. As a proof of principle, we demo

SUBMITTER: Liu JF 

PROVIDER: S-EPMC12267987 | biostudies-literature | 2025

REPOSITORIES: biostudies-literature