Predicting interacting hotspots for nanobodies' binding using triplets of residues.

ABSTRACT: Protein-protein interactions (PPI) are fundamental to cellular signaling, forming robust networks that govern critical biological processes such as immune response, cell growth, and signal transduction. Nanobody-based therapies have emerged as a key strategy for modulating PPIs, offering exceptional potential due to their high specificity, stability, and ability to access challenging epitopes on PPI interfaces inside cells. The rational design of nanobodies relies mainly on understanding and predicting their binding regions, particularly the residues that contribute the most to the binding energy (binding hotspots). Existing computational methods do not fully provide a scalable solution for hotspot identification in nanobody design, leaving a critical gap in the rational design of these th

SUBMITTER: Hamdani R 

PROVIDER: S-EPMC12268110 | biostudies-literature | 2025 Aug

REPOSITORIES: biostudies-literature