Identification of 2,3-oxidosqualene cyclase gene in <i>Eleutherococcus senticosus</i> and its regulatory mechanism in saponin synthesis.

ABSTRACT: Oleanane-type triterpenoid saponins are the primary medicinal components of Eleutherococcus senticosus. During saponin biosynthesis in E. senticosus, various members of the 2,3-oxidosqualene cyclase (OSC) gene family can direct 2,3-oxidosqualene into triterpene saponin and sterol synthesis pathways. However, the precise molecular mechanism underlying this phenomenon remains unclear. We initially screened for β-amyrin synthase 1 (bAS1) and cycloartenol synthase 1 (CAS1) among 10 EsOSC genes using genome-wide identification and correlation analysis. Subcellular localization, catalytic experiments, and in vivo transient overexpression demonstrated that EsbAS1 and EsCAS1 catalyze the formation of the triterpene skeleton β-amyrin and sterol precursor cy

SUBMITTER: Cui Y 

PROVIDER: S-EPMC12268154 | biostudies-literature | 2025 Aug

REPOSITORIES: biostudies-literature