Bulk-level maps of pioneer factor binding dynamics during the Drosophila maternal-to-zygotic transition.

ABSTRACT: Gene regulation by transcription factors (TFs) binding cognate sequences is of paramount importance. For example, the TFs Zelda (Zld) and GAGA factor (GAF) are widely acknowledged for pioneering gene activation during zygotic genome activation (ZGA) in Drosophila. However, quantitative dose/response relationships between bulk TF concentration and DNA binding, an event tied to transcriptional activity, remain elusive. Here, we map these relationships during ZGA: a crucial step in metazoan development. To map the dose/response relationship between nuclear concentration and DNA binding, we performed raster image correlation spectroscopy, a method that can measure biophysical parameters of fluorescent molecules. We found that, although Zld concentration increases during nuclear cycles 10 to 14

SUBMITTER: Dima SS 

PROVIDER: S-EPMC12268169 | biostudies-literature | 2025 Jul

REPOSITORIES: biostudies-literature