In vivo transition in chromatin accessibility during differentiation of deep-layer excitatory neurons in the neocortex.

ABSTRACT: During neuronal differentiation, gene transcription patterns change in response to both intrinsic and extrinsic cues. Chromatin regulation at regulatory elements plays a key role in this process. However, how chromatin accessibility evolves in vivo in cortical neurons remains unclear. Here, we established a method for labeling differentiating neurons with specific birthdates. Using this method, we traced the 4-day differentiation process of in vivo deep-layer excitatory neurons in the mouse embryonic cortex and examined changes in the genome-wide transcription pattern and chromatin accessibility using RNA sequencing and DNase sequencing, respectively. We found that genomic regions of genes linked to mature neuronal functions, including deep layer-specific and stimulus-responsive genes, bec

SUBMITTER: Sakai S 

PROVIDER: S-EPMC12268177 | biostudies-literature | 2025 Jul

REPOSITORIES: biostudies-literature