Temporal dynamics of gene and protein signatures following volumetric muscle loss.

ABSTRACT:

Introduction

Volumetric muscle loss (VML) is characterized by permanent tissue impairment resulting from critically-sized muscle loss. We performed time-series transcriptomic and proteomic analyses to reveal key mediators of irreversible pathological remodeling after induction of VML in mice.

Methods

The dynamics of gene and protein expression patterns were analyzed for up to 3 weeks after muscle injury.

Results

RNA Sequencing revealed transcriptional patterns that show rapid upregulation or downregulation shortly after injury, among which a subset of genes failed to return to pre-injury levels within 3 weeks after VML. Time-series analysis revealed gene clusters with sustained upregulation after 3 weeks, including those associated with extracellular matrix remodeling

SUBMITTER: Jain I 

PROVIDER: S-EPMC12268354 | biostudies-literature | 2025

REPOSITORIES: biostudies-literature