Development of a KRP-based pH-responsive drug delivery system for solid tumors.

ABSTRACT:

Background

Cell-penetrating peptides (CPPs) are considered ideal carriers for the delivery of drugs through the cell membrane barrier, with enhanced permeation and retention effects. These peptides can increase the bioavailability of drugs and reduce their side effects. In this study, we developed a polypeptide called "cell-penetrating peptide (KRP)", linked it with doxorubicin (DOX), and created a pH-responsive triggered drug delivery system called "KRP-Hyd-DOX".

Methods

We generated KRP through the application of standard Fmoc solid-phase peptide synthesis (SPPS). We attached DOX through a stable amide bond, using 3-maleimidopropionic acid as a linker, yielding KRP-DOX. With 6-maleimidopropionic acid as a linker, we connected DOX via a pH-sensitive hydrazone bond, resultin

SUBMITTER: Wu Y 

PROVIDER: S-EPMC12268649 | biostudies-literature | 2025 Jun

REPOSITORIES: biostudies-literature