Altered cofactor recruitment and nucleosome dynamics underlie bisphenol A's impact on ERα-mediated transcriptional bursting.

ABSTRACT: Transcription initiation at hormone-responsive gene promoters involves recruitment of numerous proteins that initiate stochastic events known as transcriptional bursts. Estrogen responsive genes are regulated by Estradiol (E2) that binds to the Estrogen Receptor-α (ERα), allowing ERα to interact with DNA regulatory elements, recruit cofactors, and initiate transcription. Here, we utilized single-molecule imaging to determine how ERα mediated transcription is altered by non-canonical ligands such as Bisphenol A (BPA). Our analysis showed that the TFF1 gene exhibited similar burst initiation kinetics in BPA-treated cells compared to cells treated with E2. However, there was a significant reduction in the number of active alleles in the BPA-treated cells. We show that while BPA bound E

SUBMITTER: Day CR 

PROVIDER: S-EPMC12268693 | biostudies-literature | 2025 Jul

REPOSITORIES: biostudies-literature