Precondensed Plasmid DNA Enhances CAR‑T Cell Generation via Lipid Nanoparticles.

ABSTRACT: The advent of chimeric antigen receptor (CAR) T-cell therapy has introduced a novel and personalized approach to cancer treatment. Despite its promise, the challenge of developing a system that bypasses the need for viral vectors remains significant, particularly in terms of achieving a clinical efficacy and sustained durability. To address these challenges, lipid nanoparticles (LNPs) produced through advanced microfluidic technology have been recently utilized to encapsulate plasmid DNA (pDNA) encoding CAR receptors. However, the intrinsic challenges associated with pDNA encapsulation, along with the critical requirement for efficient expression, remain substantial obstacles. Here, we show that incorporating DNA-condensing agents into the microfluidic manufacturing of LNPs effectively ove

SUBMITTER: Pirrottina A 

PROVIDER: S-EPMC12268738 | biostudies-literature | 2025 Jul

REPOSITORIES: biostudies-literature