Transcriptome sequencing showed the differential expression of circRNAs in human pulmonary microvascular endothelial cells in acute respiratory distress syndrome.

ABSTRACT:

Background

Acute respiratory distress syndrome (ARDS) is characterized by elevated pulmonary microvascular permeability; however, the role of circular RNAs (circRNAs) in this process remains unclear. Our study aims to discover the mechanism underlying the role of circRNA in pulmonary microvascular permeability in ARDS.

Methods

We developed an in vitro model of ARDS using cultured human pulmonary microvascular endothelial cells (HPMECs) and lipopolysaccharide challenge. Genome sequencing revealed significant differences among the cells in the expression of circRNA. Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) analyses of the target genes were conducted. A circRNA-microRNA (miRNA)-messenger RNA (mRNA) competitive endogenous RNA (ceRNA) network w

SUBMITTER: Wang N 

PROVIDER: S-EPMC12268750 | biostudies-literature | 2025 Jun

REPOSITORIES: biostudies-literature