Itaconate suppresses neonatal intestinal inflammation via metabolic reprogramming of M1 macrophage.

ABSTRACT:

Background

Necrotizing enterocolitis (NEC) is a rapidly progressive and severe gastrointestinal disorder in neonates that is marked by an inflammatory cascade initiated by mechanisms that remain incompletely understood, resulting in intestinal necrosis and systemic infections. This study demonstrated that itaconate (ITA) exerts a protective effect in NEC by regulating macrophage reprogramming.

Methods

Changes in ITA expression were investigated using immunofluorescence staining and liquid chromatography-mass spectrometry, and their effect on immune cell differentiation was verified through single-cell sequencing. In vivo experiments were performed using ACOD1^-/- and ACOD1^fl/flLysM^cre NEC mouse models.

Results

We detected changes in ITA

SUBMITTER: Huangfu S 

PROVIDER: S-EPMC12268792 | biostudies-literature | 2025 Jul

REPOSITORIES: biostudies-literature