Smart-Plexer 2.0: Leveraging New Features of Amplification Curves to Enhance the Selection of Multiplex PCR Assays in Multi-Target Identification.

ABSTRACT: Multiplex PCR plays a critical role in diagnostics by enabling the detection of multiple targets in a single reaction. However, its use is often limited by the need for multiple fluorescent channels, which are restricted in standard PCR instrumentation. Amplification curve analysis (ACA) is a data-driven multiplexing (DDM) approach that overcomes this limitation by using real-time PCR data and machine learning to differentiate targets in a single-channel, single-well format, without requiring instrument modifications. As part of this DDM strategy, we previously introduced Smart-Plexer 1.0, a tool that simulates multiplex assays using empirical singleplex data to identify optimal assay combinations in silico, maximizing kinetic feature distances between targets to support ACA-based discrimi

SUBMITTER: Xu K 

PROVIDER: S-EPMC12268814 | biostudies-literature | 2025 Jul

REPOSITORIES: biostudies-literature