<i>Pseudomonas putida</i> Coordinates the Expression of two Manganese Oxidases and Optimizes Manganese Oxide Precipitation in Response to Aqueous Mn(II).

ABSTRACT: Manganese (Mn) oxides are commonly precipitated by bacteria and fungi. Recent studies have shown that Mn-oxidizing bacteria can harbor multiple Mn oxidases, but environmental controls on the regulation of these enzymes are unknown. Here, we examine the activation of the genes encoding for MnxG and McoA, two Mn oxidases in Pseudomonas putida GB-1, in response to varying Mn(II) concentrations. Using reporter gene fusion strains, we found that mnxG and mcoA are activated only by a fraction of the cells in the population and at different threshold Mn concentrations, with an increasing proportion of cells expressing mnxG (<10 μM) and then mcoA (>10 to 500 μM). Kinetic modeling showed that coexpression of mnxG and mcoA not only alleviates substrat

SUBMITTER: Gehin G 

PROVIDER: S-EPMC12269067 | biostudies-literature | 2025 Jul

REPOSITORIES: biostudies-literature