The Clumped Isotope Signatures of Multiple Methanogenic Metabolisms.

ABSTRACT: Methane is a potent greenhouse gas, an important energy source, and an important part of the global carbon cycle. The relative abundances of doubly substituted ("clumped") methane isotopologues (¹³CH₃D and ¹²CH₂D₂) offer important information on the sources and sinks of methane. However, the clumped isotope signatures of microbially produced methane from different methanogenic pathways lack a systematic investigation. In this study, we provide a data set encompassing isotopic signatures of hydrogenotrophic, methylotrophic, acetoclastic, and methoxydotrophic methanogenesis. We find that a statistical "combinatorial effect" generates significant differences in ¹²CH₂D₂ compositions between hydrogenotrophic

SUBMITTER: Li J 

PROVIDER: S-EPMC12269085 | biostudies-literature | 2025 Jul

REPOSITORIES: biostudies-literature