Quantitative analysis of ethylene precursor ACC in plant samples by liquid chromatography-tandem mass spectrometry.

ABSTRACT:

Background

Ethylene (C₂H₄) is a gaseous phytohormone that regulates various plant physiological processes and mediates the responses of the plants to various environmental stresses. 1-Aminocyclopropane-1-carboxylic acid (ACC) is the direct precursor of the phytohormone ethylene, and is also considered as a plant growth regulator. Accurate quantification of ACC is critically important in investigating its function. However, it remains challenging to accurately quantify ACC in plant tissues because it is a small, electroneutral molecule with very low concentrations.

Methods

An easy, cost-saving, and highly efficient quantitative method for ACC in plant tissues was set up by liquid-liquid micro-extraction (LLME) purification with green solvent ethyl-acetat

SUBMITTER: Tong J 

PROVIDER: S-EPMC12269232 | biostudies-literature | 2025 Jul

REPOSITORIES: biostudies-literature