Campylobacter jejuni regulates cell cycle progression to potentiate host cell invasion.

ABSTRACT:

Background

Campylobacter jejuni is associated with enteritis in humans and domestic animals. Acute C. jejuni-mediated enteritis requires bacterial invasion of intestinal cells with an ensuing host inflammatory response. Known is that C. jejuni invasion of human epithelial cells is dependent on host cell-focal adhesion components, which link the extracellular matrix to the actin cytoskeleton of a cell. Based on the observation that C. jejuni cell invasion engages many of the same components involved in regulating the cell cycle, we hypothesized that C. jejuni regulates the host cell cycle.

Methods

Flow cytometry was used to detect the cell cycle phases (G₁, S, G₂ and M). Single-cell RNA-sequencing (scRNA-seq) and reverse transcriptase quantitative PCR (R

SUBMITTER: Talukdar PK 

PROVIDER: S-EPMC12269301 | biostudies-literature | 2025 Jul

REPOSITORIES: biostudies-literature