Deletion of a polyketide synthase <i>phoE</i> induces monoterpenes production in ascidian-derived fungus <i>Diaporthe</i> sp. SYSU-MS4722.

ABSTRACT: Marine-derived fungi represent an underexplored source of monoterpenes, with only sporadic reports over the past two decades, making their discovery particularly challenging. Whole-genome sequencing of Diaporthe sp. SYSU-MS4722, an ascidian-derived fungus isolated from Styela plicata, identified 130 biosynthetic gene clusters (BGCs), underscoring its vast biosynthetic potential. However, previous studies demonstrated that Diaporthe sp. SYSU-MS4722 predominantly produces xanthone phomoxanthone A under standard laboratory conditions, suggesting that many BGCs remain cryptic. Notably, 16 of these BGCs encode terpene synthase genes, indicating the potential for monoterpene biosynthesis. To activate these silent BGCs and discover monoterpenes, we deleted the polyketide synt

SUBMITTER: Yuan S 

PROVIDER: S-EPMC12269604 | biostudies-literature | 2025

REPOSITORIES: biostudies-literature