Map of the neuronal O-glycoproteome reveals driver functions in the regulated secretory pathway.

ABSTRACT: Impairments in protein glycosylation, including O-GalNAc-type glycosylation, are linked to severe developmental disorders with prominent neurological involvement. However, the role of this glycosylation pathway at a cellular level is not yet fully understood. Here, we report a comprehensive map of GalNAc-type O-glycoproteins (>800) and O-glycosites (>4000) from neuronal tissues and cell lines and identify abundant O-glycosites within major classes of proteins involved in neuroplasticity, including axon guidance, membrane remodeling, and regulated vesicular secretion. Applying the map, we demonstrate that the regulated secretory pathway constitutes highly O-glycosylated proteins including Chromogranin A, a key player in dense core granulogenesis, and that correct O-glycosylation is importan

SUBMITTER: Madsen TD 

PROVIDER: S-EPMC12269846 | biostudies-literature | 2025 Jul

REPOSITORIES: biostudies-literature