Species-level enumeration of low-concentration lactic acid bacteria in breast milk.

ABSTRACT: It is crucial to identify the distribution of lactic acid bacteria (LAB) in breast milk which is a source of live probiotics for the infant gut. Quantitative PCR (qPCR) is a powerful technique for selectively counting LAB at the species or strain level. However, the limit of quantification (LoQ) of qPCR is inadequate for human milk samples. To address this issue, nested primer pairs for Lactobacillus and Bifidobacterium species were designed to enable 15 cycles of preamplification Subsequent qPCR assays using the preamplification products (with 10-fold dilution) as templates reduced the LoQ for enumeration plasmids to one-thousandth of the original concentration. Importantly, preamplification did not enhance the detection capability for low biomass samples because the efficie

SUBMITTER: Sun Y 

PROVIDER: S-EPMC12270009 | biostudies-literature | 2025 Dec

REPOSITORIES: biostudies-literature