The quantitative detection method employing a combination of high-affinity antibodies targeting PCT.

ABSTRACT:

Background and aims

Procalcitonin (PCT) is a widely recognized inflammation marker utilized in various clinical testing contexts and is subject to ongoing refinements, thereby imposing greater demands on core antibodies. However, the published literature lacks a comprehensive description of them.

Material and methods

In this study, we initially expressed the full-length PCT protein in eukaryotic systems, followed by conventional antibody engineering techniques for in vitro cell fusion and the selection of positive hybridoma cell clones specific to the PCT protein.

Results

A total of 83 positive clones were generated, among which 15 high-affinity IgG₁ subtype monoclonal antibodies were selected for complementarity-determining region (CDR) analysis to

SUBMITTER: Cheng X 

PROVIDER: S-EPMC12270020 | biostudies-literature | 2025 Sep

REPOSITORIES: biostudies-literature