Reverse-normal immunopurification: An effective approach for purifying recombinant erythropoietin from its analogues in doping analysis.

ABSTRACT:

Background

Recombinant erythropoietin (rEPO) is commonly used in therapy but may be abused in sports to enhance endurance. In doping analysis, rEPO can be detected in human urine or blood samples at picogram (pg) levels based on its slightly higher molecular weight (MW) than that of endogenous EPO using western blotting (WB). However, a type of variant erythropoietin (VAR-EPO) encoded by the EPO c.577del variant has a similar MW to rEPO, and these 2 molecules cannot be distinguished using conventional analytical methods. A fit-for-purpose method needs to be developed immediately.

Methods

In this study, we introduced a reverse-normal immunopurification technique for sample pretreatment to remove VAR-EPO from samples to eliminate its interference with rEPO detection. Firstly,

SUBMITTER: He S 

PROVIDER: S-EPMC12270052 | biostudies-literature | 2025 May

REPOSITORIES: biostudies-literature