Bradykinin measurement by liquid chromatography tandem mass spectrometry in subjects with hereditary angioedema enhanced by cold activation.

ABSTRACT:

Background

Bradykinin (BK), a 9-amino acid peptide, is a key mediator responsible for angioedema attacks in hereditary angioedema due to C1INH deficiency (HAE-C1INH). Current BK assays lack clinical utility due to the instability of BK (half-life < 30 seconds) and low pg/mL baseline levels.

Objective

We sought to develop a novel method to overcome the issues in current protease inhibitor-based methods for measuring endogenous BK.

Methods

Blood samples from subjects with HAE-C1INH and healthy volunteers were collected and subjected to cold activation for the contact system. Cold-induced BK was measured via LC-MS/MS. The protocol was developed and validated according to the US Food and Drug Administration Bioanalytical Method Validation guidelines. The procedure was opt

SUBMITTER: Chen J 

PROVIDER: S-EPMC12270067 | biostudies-literature | 2025 Aug

REPOSITORIES: biostudies-literature