A Simple Strategy for Co-Immobilization of NADH-Dependent Enzymes and NADH for Efficient Biocatalysis in Continuous Flow Reactors.

ABSTRACT: The application of nicotinamide adenine dinucleotide (NAD)(H)-dependent enzymes in continuous flow reactors poses significant challenges due to the high cost of NAD(H) and its inevitable loss during reactor operation. To overcome these limitations, a novel platform comprising Cry3Aa-enzyme fusion particles modified with polyethylenimine (PEI) is developed and its application for two catalytic processes is demonstrated. In the first system, two NAD(H)-dependent enzymes, formate dehydrogenase (FDH), and leucine dehydrogenase (LDH) are genetically co-immobilized within Cry3Aa particles and then modified with PEI to facilitate NADH co-immobilization. To adapt the system for continuous flow reactors, PEI-modified particles are entrapped within agarose beads, loaded with NADH, and then used to c

SUBMITTER: Yekta R 

PROVIDER: S-EPMC12270371 | biostudies-literature | 2025 Jul

REPOSITORIES: biostudies-literature