Impact of cell culture transition on adipose-derived mesenchymal stromal cells: Multi-omics analysis of small- vs large-scale production.

ABSTRACT: Mesenchymal stromal cells (MSCs) are being tested in numerous clinical trials, yet the limited progression of these trials to advanced stages indicates unresolved translational challenges. Expanding MSCs is a critical step in most therapeutic applications, and bioreactor-based culture offers large-scale production compared to monolayer cultures. Nevertheless, since MSCs sense their microenvironment, it is crucial to understand how these systems affect their properties. We expanded human adipose-derived mesenchymal stromal cells (AD-MSCs) from the same donors in both small- and large-scale platforms. Bulk-RNA sequencing (RNA-seq) and mass spectrometry analysis demonstrated that small-scale culture had a broader range of differentially expressed genes (DEGs) and proteins within immunomodulat

SUBMITTER: Galera MR 

PROVIDER: S-EPMC12270734 | biostudies-literature | 2025 Sep

REPOSITORIES: biostudies-literature