Intraline genomic heterogeneity of the triple-negative breast cancer MDA-MB-231-luc-GFP cell line.

ABSTRACT: Cancer cell lines are valuable models for studying tumor biology, yet their genomic evolution during culture can compromise experimental reproducibility. We conducted a detailed genomic analysis of the triple-negative breast cancer cell line MDA-MB-231-luc-GFP, examining sublines obtained from different sources, at various time points, and across distinct passages. We introduce the concept of intraline heterogeneity (ILH) to highlight the genomic variability observed among these sublines. Our analyses revealed extensive genomic diversity, including differences in single-nucleotide variants (SNVs) and copy number alterations (CNAs). In particular, CNAs exhibited remarkable heterogeneity, with pronounced chromosomal gains and losses between sublines, underscoring the impact of genomic instab

SUBMITTER: Varela-Rouco N 

PROVIDER: S-EPMC12270797 | biostudies-literature | 2025

REPOSITORIES: biostudies-literature