Ultrasound-assisted deep eutectic solvent of neuroprotective extracts from Paeonia lactiflora Pall. root: Process optimization, compositions characterization, and neuroprotective activity validation.

ABSTRACT: The objective of this study was to efficiently extract components with neuroprotective activity from the root of Paeonia lactiflora Pall. (RPL). The neuroprotective extracts of RPL were extracted by ultrasound-assisted deep eutectic solvents (DESs) method, and optimized by response surface methodology with PC12 cell viability as a guide. The optimal process: DESs (composed of L-Proline and Glycerol, Betaine monohydrate and Benzyl alcohol in a ratio of 7:3 (v/v) with 60 % water), extraction time 22 min, liquid-to-solid ratio 42 mL/g, temperature 51 °C, ultrasonic power 243 W. These conditions yielded extracts with cell viability 81.44 ± 0.18 % (n = 3), which is much higher than conventional heat and standard DESs extraction methods. Then, the RPL neuroprotective purified extract (RNPE) was

SUBMITTER: Xue Y 

PROVIDER: S-EPMC12270807 | biostudies-literature | 2025 Jun

REPOSITORIES: biostudies-literature