Targeting revascularization via tryptophan-indole-NETs axis: the synergistic power of acupuncture and rt-PA thrombolysis in ischemic stroke.

ABSTRACT:

Background

The Xingnao kaiqiao (XNKQ) method, meaning "awakening the mind," is an acupuncture technique developed by Academician Xuemin Shi. It has shown promise in ischemic stroke management. This study aims to explore the synergistic power and underlying mechanisms of XNKQ method and recombinant tissue plasminogen activator (rt-PA).

Methods

A rat embolic stroke model was utilized to replicate the pathological processes of ischemic stroke. The effects on cerebral perfusion and vascular leakage were assessed in vivo and in vitro. Both non-targeted and tryptophan-targeted metabolomic analyses were conducted to identify differential metabolites. Additionally, neutrophil extracellular traps (NETs) markers and vascular leakage were detected to elucidate the crossta

SUBMITTER: Chang S 

PROVIDER: S-EPMC12271142 | biostudies-literature | 2025

REPOSITORIES: biostudies-literature