A promoter library for tuning gene expression in <i>Cupriavidus necator</i> under autotrophic conditions.

ABSTRACT: Cupriavidus necator holds promise for biomanufacturing using CO₂ as the primary feedstock, leveraging its capabilities to produce valuable chemicals and grow autotrophically using H₂ as an energy source. Although various genetic tools, including promoters, have been developed to fine-tune gene expression in C. necator, no such tools have been developed for the use in autotrophic conditions. This study aimed to establish a promoter library that functions in C. necator grown under autotrophic conditions. C. necator was cultured under both heterotrophic and autotrophic conditions, and comparative transcriptome analysis was performed to identify genes/operons specifically upregulated under autotrophic conditions and those constitutively express

SUBMITTER: Kitagawa W 

PROVIDER: S-EPMC12271211 | biostudies-literature | 2025

REPOSITORIES: biostudies-literature