An optimised promoter and signal peptide improves methionine production of a genetically engineered <i>Candida utilis</i> harboring the <i>δ-zein</i> gene.

ABSTRACT:

Introduction

L-methionine is nutritionally indispensable for humans and animals. It is widely applied to feed, livestock and poultry breeding, food, medicine, energy and chemical industries. Maize endosperm contains a stable protein called δ-zein, which is abundant in sulfur amino acids, including methionine. Candida utilis (C. utilis) has been utilized as a cell factory to express and produce recombinant products. However, there is limited information on its genetic background and expression regulatory elements.

Methods

In this study, we aimed to improve methionine yields in an engineered C. utilis harboring the δ-zein gene by identifying a strong promoter and optimal signal peptide. A C. utilis glyceraldehyde-3-phosphate dehydrogen

SUBMITTER: He Q 

PROVIDER: S-EPMC12271212 | biostudies-literature | 2025

REPOSITORIES: biostudies-literature