AI-guided discovery and optimization of antimicrobial peptides through species-aware language model.

ABSTRACT: The rise of antibiotic-resistant bacteria drives an urgent need for novel antimicrobial agents. Antimicrobial peptides (AMPs) show promise solutions due to their multiple mechanisms of action and reduced propensity for resistance development. This study introduces LLAMP (Large Language model for AMP activity prediction), a target species-aware AI model that leverages pre-trained language models to predict minimum inhibitory concentration values of AMPs. Using LLAMP, we screened approximately 5.5 million peptide sequences, identifying peptides 13 and 16 as the most selective and most potent candidates, respectively. Analysis of attention values allowed us to pinpoint critical amino acid residues (e.g., Trp, Lys, and Phe). Using the critical amino acids, the sequence of the most selective pe

SUBMITTER: Bae D 

PROVIDER: S-EPMC12271573 | biostudies-literature | 2025 Jul

REPOSITORIES: biostudies-literature