MicroRNA-responsive ON-OFF hybrid mRNA switch for precise protein expression control.

ABSTRACT: Messenger RNA (mRNA) technology is gaining attention for its potential in developing next-generation therapeutics. However, developing effective protein expression control technologies to regulate mRNA translation, particularly in a cell type- and tissue-specific manner, remains challenging. To achieve enhanced precision in translational regulation, we developed a microRNA (miRNA)-sensing hybrid mRNA switch, integrating an ON-type switch, which activates translation by detecting specific miRNAs expressed in target cells, into an OFF-type switch, which represses translation by sensing another miRNA in non-target cells. We first investigated effective module sequences after the poly(A)-tail for the ON-type mRNA switch to ensure appropriate translational activation in response to target cell-

SUBMITTER: Masaki K 

PROVIDER: S-EPMC12271581 | biostudies-literature | 2025 Sep

REPOSITORIES: biostudies-literature