Engineering <i>Yarrowia lipolytica</i> for high-level β-elemene production via combinatorial metabolic strategies.

ABSTRACT: β-Elemene, a pharmacologically active sesquiterpene derived from the traditional Chinese medicinal herb Curcuma wenyujin, exhibits broad-spectrum anti-tumor and anti-inflammatory activities. Currently, its commercial production relies heavily on plant extraction, a process associated with high costs and environmental burdens, limiting industrial scalability. To address these challenges, we developed a metabolically engineered Yarrowia lipolytica strain as an efficient microbial cell factory for de novo Germacrene A biosynthesis, the direct precursor of β-elemene. Through combinatorial optimization strategies-including (1) mevalonate (MVA) pathway enhancement, (2) copy number amplification of germacrene A synthase (GAS), (3) β-oxidation pathway reinforcement, and (4) introduct

SUBMITTER: Dai M 

PROVIDER: S-EPMC12271781 | biostudies-literature | 2025 Dec

REPOSITORIES: biostudies-literature