Controlling Topology of a Telomeric G-quadruplex DNA With a Chemical Cross-link.

ABSTRACT: DNA G-quadruplexes (G4s) are noncanonical structures formed in guanine-rich sequences. Within the human genome, they are nonrandomly distributed and influence DNA replication, gene expression, and genome maintenance. Numerous proteins involved in these processes have been identified as G4-binding proteins. However, the interaction of proteins with G4s in the context of double-stranded DNA in vitro has been difficult to study due to the transient nature of G4s in the presence of complementary DNA. To overcome this challenge, introducing internal covalent cross-links between distant nucleotides within the DNA sequence may promote pre-folding of G4 structures, thereby shifting the thermodynamic equilibrium toward G4-formation. We used a Cu(I)-catalyzed azide-alkyne cycloaddition to create a c

SUBMITTER: Chilton B 

PROVIDER: S-EPMC12272027 | biostudies-literature | 2025 Jul

REPOSITORIES: biostudies-literature