Quantification of [¹¹C]ABP688 Binding to mGluR5 in Human Brain using Cerebellum as Reference Region: Biological Interpretation and Limitations.

ABSTRACT:

Introduction

In vitro data from primates provide conflicting evidence about the suitability of the cerebellum as a reference region for quantifying type 5 metabotropic glutamate receptor (mGluR5) binding parameters with positron emission tomography (PET).

Methods

We first measured mGluR5 density in postmortem human cerebellum using [³H]ABP688 autoradiography (n=5) and immunohistochemistry (n=6). Next, in vivo experiments were conducted in healthy volunteers (n=6) using a high-resolution PET scanner (HRRT) to compare [¹¹C]ABP688 binding potential (BPND) values obtained with reference tissue methods and the two-tissue compartment model vs. metabolite-corrected arterial input function.

Results

The postmortem data showed that, relative to the

SUBMITTER: Milella MS 

PROVIDER: S-EPMC12272086 | biostudies-literature | 2025

REPOSITORIES: biostudies-literature