Systematic screening of excipients to stabilize aerosolized lipid nanoparticles for enhanced mRNA delivery.

ABSTRACT: Aerosolized lipid nanoparticles (LNPs) delivering mRNA are an attractive strategy for use in local, inhalable therapy to treat patients with lung diseases. However, a major barrier to delivering aerosolized mRNA LNPs is the shear forces encountered during aerosolization. These forces lead to significant morphology changes and subsequent decrease in efficacy of mRNA delivery. To best retain the physicochemical properties of mRNA LNPs during aerosolization, we took a formulation-based strategy to stabilize LNPs. We used a design-of-experiment (DOE) approach to comprehensively screen rationally chosen excipients at multiple concentrations. Excipients were carefully selected based on their use in clinically approved inhaled products or their ability to support lipid membrane properties. These

SUBMITTER: Heiser BJ 

PROVIDER: S-EPMC12272335 | biostudies-literature | 2025 Sep

REPOSITORIES: biostudies-literature