Exploring LAT-derived miRNAs as regulatory agents of EphrinA3 in glioblastoma multiforme.

ABSTRACT:

Background

This study focuses on the findings related to Latency- Associated Transcript (LAT)- derived miRNAs and their interactions with ephrin family genes, especially EFNA3. It contextualizes, these results within the broader Glioblastoma multiform (GBM) research landscape.

Method

The differential expression of the Ephrin (EFN) family in GBM was analyzed using TCGA and GEO databases, alongside survival data from the Kaplan-Meier Plotter. Bioinformatics predicted LAT-derived miRNAs targeting EFN genes, which were validated in vitro. Luciferase assays confirmed miR-H2 and miR-H3's targeting of EFNA3, while qRT-PCR and Western blotting assessed their effects on mRNA and protein levels.

Results

EphrinA3 is significantly overexpressed in GBM tissues, and i

SUBMITTER: Saadatpour F 

PROVIDER: S-EPMC12272618 | biostudies-literature | 2025 Sep

REPOSITORIES: biostudies-literature